PrimePCR™ SYBR® Green Assay: Dlg2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Dlg2, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Dlg2, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCID0006766
Assay Design:		intron_spanning
Chromosome Location:		1:163213324-163369450question
Amplicon Length:		135
Splice Variants Targeted:		ENSRNOT00000066122 ENSRNOT00000007768 ENSRNOT00000044234 ENSRNOT00000055401 ENSRNOT00000055402 ENSRNOT00000034416 ENSRNOT00000055397 ENSRNOT00000055399

Gene Information

membrane associated guanylate kinase localized at postsynaptic density (PSD); may function to maintain the structure of PSD by concentrating its components to the membrane area [RGD, Feb 2006]

Gene Symbol:		Dlg2
Gene Name:		Disks large homolog 2
Aliases:		Not Available
RefSeq:		NM_022282
Ensembl:		ENSRNOG00000022635
Entrez:		64053
Chromosome Mapping:		1q32

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	36.100000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.