PrimePCR™ SYBR® Green Assay: Pla2g4a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0008045
Assay Design:   intron_spanning
Chromosome Location:   13:72050811-72056530question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000003630

Gene Information

catalyzes the synthesis of COX-2 substrate arachidonic acid (AA); may play a role in epileptogenesis [RGD, Feb 2006]

Gene Symbol:   Pla2g4a
Gene Name:   Cytosolic phospholipase A2
Aliases:   Not Available
RefSeq:   NM_133551
Ensembl:   ENSRNOG00000002657
Entrez:   24653
Chromosome Mapping:   13q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.380000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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