PrimePCR™ SYBR® Green Assay: Myh9, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Myh9, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Myh9, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0015547
Assay Design:   intron_spanning
Chromosome Location:   7:119034250-119039021question
Amplicon Length:   105
Splice Variants Targeted:   ENSRNOT00000020028 ENSRNOT00000005581 ENSRNOT00000000497 ENSRNOT00000034400 ENSRNOT00000044317 ENSRNOT00000001401 ENSRNOT00000007229 ENSRNOT00000068192 ENSRNOT00000031906 ENSRNOT00000016196 ENSRNOT00000007398 ENSRNOT00000037611 ENSRNOT00000075011 ENSRNOT00000026838 ENSRNOT00000010264 ENSRNOT00000024246

Gene Information

cellular myosin; involved in cytokinesis [RGD, Feb 2006]

Gene Symbol:   Myh9
Gene Name:   Myosin, heavy polypeptide 9, non-muscle; Myosin-9
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049236
Entrez:   25745
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download