PrimePCR™ SYBR® Green Assay: Ebna1bp2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ebna1bp2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ebna1bp2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0016821
Assay Design:   intron_spanning
Chromosome Location:   5:141457830-141458845question
Amplicon Length:   99
Splice Variants Targeted:   ENSRNOT00000035100 ENSRNOT00000028786 ENSRNOT00000022324 ENSRNOT00000064945 ENSRNOT00000047596 ENSRNOT00000009203 ENSRNOT00000075464 ENSRNOT00000072245 ENSRNOT00000035199 ENSRNOT00000022282 ENSRNOT00000055279 ENSRNOT00000036834 ENSRNOT00000004163 ENSRNOT00000067889 ENSRNOT00000024253 ENSRNOT00000071485 ENSRNOT00000036770

Gene Information

Description Not Available

Gene Symbol:   Ebna1bp2
Gene Name:   Probable rRNA-processing protein EBP2
Aliases:   Not Available
RefSeq:   NM_001008721
Ensembl:   ENSRNOG00000045760
Entrez:   114021
Chromosome Mapping:   5q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.890000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download