PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000047715, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000047715, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000047715, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0020587
Assay Design:   intron_spanning
Chromosome Location:   1:221970891-221981229question
Amplicon Length:   173
Splice Variants Targeted:   ENSRNOT00000014004 ENSRNOT00000025394 ENSRNOT00000002292 ENSRNOT00000018982 ENSRNOT00000008055 ENSRNOT00000011207 ENSRNOT00000073221 ENSRNOT00000013577 ENSRNOT00000051307 ENSRNOT00000024196 ENSRNOT00000064212 ENSRNOT00000049064 ENSRNOT00000035541 ENSRNOT00000017112 ENSRNOT00000061735 ENSRNOT00000001826 ENSRNOT00000061068 ENSRNOT00000031118 ENSRNOT00000045827 ENSRNOT00000005352 ENSRNOT00000024030 ENSRNOT00000064910 ENSRNOT00000003349 ENSRNOT00000056735 ENSRNOT00000071031 ENSRNOT00000010683 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000047715
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047715
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.710000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download