PrimePCR™ SYBR® Green Assay: OAZ1, Pig

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: OAZ1, Pig
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: OAZ1, Pig
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qSscCED0015772
Assay Design:   Exonic
Chromosome Location:   2:76855268-76855358question
Amplicon Length:   61
Splice Variants Targeted:   ENSSSCT00000014704

Gene Information

The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamine in cells. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis pathway; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as by inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs, and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization (PMID:16120325). Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jul 2014]

Gene Symbol:   OAZ1
Gene Name:   Sus scrofa ornithine decarboxylase antizyme 1 (OAZ1), mRNA.
Aliases:   Not Available
RefSeq:   NM_001122994
Ensembl:   ENSSSCG00000013460
Entrez:   397034
Chromosome Mapping:   2

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download