PrimePCR™ SYBR® Green Assay: EIF4A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040831

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EIF4A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EIF4A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038108
Assay Design:   exonic
Chromosome Location:   17:7477877-7478454question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000582169 ENST00000578495 ENST00000293831 ENST00000585024 ENST00000583802 ENST00000577269 ENST00000584784 ENST00000582746 ENST00000584860 ENST00000581770 ENST00000581384 ENST00000577929 ENST00000380512

Gene Information

Description Not Available

Gene Symbol:   EIF4A1
Gene Name:   eukaryotic translation initiation factor 4A1
Aliases:   DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I
RefSeq:   NC_000017.10 NG_009204.1 NT_010718.16
Ensembl:   ENSG00000161960
Entrez:   1973
Chromosome Mapping:   17p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.650000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download