PrimePCR™ SYBR® Green Assay: TAB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027485

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TAB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TAB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010189
Assay Design:   Intron-spanning
Chromosome Location:   6:149691193-149699264question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000367456 ENST00000538427 ENST00000286332 ENST00000392282

Gene Information

The protein encoded by this gene is an activator of MAP3K7/TAK1 which is required for for the IL-1 induced activation of nuclear factor kappaB and MAPK8/JNK. This protein forms a kinase complex with TRAF6 MAP3K7 and TAB1 thus serves as an adaptor linking MAP3K7 and TRAF6. This protein TAB1 and MAP3K7 also participate in the signal transduction induced by TNFSF11/RANKl through the activation of the receptor activator of NF-kappB (TNFRSF11A/RANK) which may regulate the development and function of osteoclasts. [provided by RefSeq Jul 2008]

Gene Symbol:   TAB2
Gene Name:   TGF-beta activated kinase 1/MAP3K7 binding protein 2
Aliases:   CHTD2, FLJ21885, KIAA0733, MAP3K7IP2
RefSeq:   NC_000006.11 NG_012301.1 NG_021386.1 NT_025741.15
Ensembl:   ENSG00000055208
Entrez:   23118
Chromosome Mapping:   6q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.590000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download