PrimePCR™ SYBR® Green Assay: TNFRSF18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028021

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TNFRSF18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TNFRSF18, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011446
Assay Design:   Intron-spanning
Chromosome Location:   1:1139817-1140864question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000328596 ENST00000379268 ENST00000379265

Gene Information

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq Feb 2011]

Gene Symbol:   TNFRSF18
Gene Name:   tumor necrosis factor receptor superfamily, member 18
Aliases:   AITR, GITR, GITR-D
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000186891
Entrez:   8784
Chromosome Mapping:   1p36.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download