PrimePCR™ SYBR® Green Assay: KIR2DL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040234

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIR2DL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIR2DL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037511
Assay Design:   Intron-spanning
Chromosome Location:   19:55253687-55255337question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000400848 ENST00000344867 ENST00000400875 ENST00000400847 ENST00000400829 ENST00000400827 ENST00000535453 ENST00000233607 ENST00000238483 ENST00000203556 ENST00000593186 ENST00000391728 ENST00000326542 ENST00000358178 ENST00000402254 ENST00000538269 ENST00000541392 ENST00000291860 ENST00000342376 ENST00000434419 ENST00000336077 ENST00000291633 ENST00000326321 ENST00000270442 ENST00000463876 ENST00000334661 ENST00000243776 ENST00000535926 ENST00000574960 ENST00000571420 ENST00000574061 ENST

Gene Information

Description Not Available

Gene Symbol:   KIR2DL3
Gene Name:   killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000243772
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.080000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download