PrimePCR™ SYBR® Green Assay: Myc, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0028153

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Myc, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Myc, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0006528
Assay Design:   Intron-spanning
Chromosome Location:   15:61988224-61989409question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000161976 ENSMUST00000022971 ENSMUST00000159327 ENSMUST00000167731 ENSMUST00000160009

Gene Information

The protein encoded by this gene is a multifunctional nuclear phosphoprotein that plays a role in cell cycle progression apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations overexpression rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors leukemias and lymphomas including Burkitt lymphoma in human. There is evidence to show that alternative translation initiations from an upstream in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini in human and mouse. Under conditions of stress such as high cell densities and methionine deprivation there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2010]

Gene Symbol:   Myc
Gene Name:   Myelocytomatosis oncogene
Aliases:   AU016757, Myc2, Niard, Nird, bHLHe39
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000022346
Entrez:   17869
Chromosome Mapping:   15 D2-D3|15 32.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.700000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download