PrimePCR™ SYBR® Green Assay: MAX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: MAX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAX, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005228
Assay Design:   Exonic
Chromosome Location:   14:65544650-65544740question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000358402 ENST00000284165 ENST00000358664 ENST00000555419 ENST00000557277 ENST00000556892 ENST00000556979 ENST00000555667 ENST00000557746 ENST00000556443 ENST00000441116

Gene Information

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members which include Mad Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. [provided by RefSeq Jul 2008]

Gene Symbol:   MAX
Gene Name:   MYC associated factor X
Aliases:   MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000125952
Entrez:   4149
Chromosome Mapping:   14q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download