PrimePCR™ SYBR® Green Assay: HIF1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050075

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIF1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIF1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042813
Assay Design:   exonic
Chromosome Location:   14:62207736-62207876question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000337138 ENST00000394997 ENST00000323441 ENST00000557538 ENST00000539097

Gene Information

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1) which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes including those involved in energy metabolism angiogenesis apoptosis and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   HIF1A
Gene Name:   hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Aliases:   HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100644
Entrez:   3091
Chromosome Mapping:   14q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.790000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download