PrimePCR™ SYBR® Green Assay: RHEB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050424

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RHEB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RHEB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043162
Assay Design:   exonic
Chromosome Location:   7:151164277-151167735question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000262187 ENST00000472642 ENST00000496004

Gene Information

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped two on chromosome 10 and one on chromosome 22. [provided by RefSeq Jul 2008]

Gene Symbol:   RHEB
Gene Name:   Ras homolog enriched in brain
Aliases:   MGC111559, RHEB2
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000106615
Entrez:   6009
Chromosome Mapping:   7q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download