PrimePCR™ SYBR® Green Assay: PLA2G4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050960

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLA2G4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLA2G4A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043698
Assay Design:   exonic
Chromosome Location:   1:186957522-186957625question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000367466 ENST00000442353

Gene Information

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids including prostaglandins and leukotrienes are lipid-based cellular hormones that regulate hemodynamics inflammatory responses and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq Jul 2008]

Gene Symbol:   PLA2G4A
Gene Name:   phospholipase A2, group IVA (cytosolic, calcium-dependent)
Aliases:   MGC126350, PLA2G4, cPLA2-alpha
RefSeq:   NC_000001.10 NG_012203.1 NT_004487.19
Ensembl:   ENSG00000116711
Entrez:   5321
Chromosome Mapping:   1q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download