PrimePCR™ SYBR® Green Assay: INCENP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052714

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: INCENP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: INCENP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045453
Assay Design:   exonic
Chromosome Location:   11:61896328-61897326question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000394818 ENST00000533896 ENST00000278849

Gene Information

In mammalian cells 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' or transiently interacting proteins (reviewed by Choo 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139) CENPB (MIM 117140) CENPC1 (MIM 117141) and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g. MIM 600112); CliPs (e.g. MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke 1991 [PubMed 1860899]) the initial members of the passenger protein group display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase the proteins are located within the midbody in the intercellular bridge where they are discarded after cytokinesis (Cutts et al. 1999 [PubMed 10369859]).[supplied by OMIM Mar 2008]

Gene Symbol:   INCENP
Gene Name:   inner centromere protein antigens 135/155kDa
Aliases:   FLJ31633, MGC111393
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000149503
Entrez:   3619
Chromosome Mapping:   11q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.590000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download