PrimePCR™ SYBR® Green Assay: CANX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027638

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CANX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CANX, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010564
Assay Design:   Intron-spanning
Chromosome Location:   5:179135292-179136033question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000510810 ENST00000508787 ENST00000415618 ENST00000376953 ENST00000354394 ENST00000512607 ENST00000506654 ENST00000502673 ENST00000452673 ENST00000502498 ENST00000247461 ENST00000504734

Gene Information

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   CANX
Gene Name:   calnexin
Aliases:   CNX, FLJ26570, IP90, P90
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000127022
Entrez:   821
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.390000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download