PrimePCR™ SYBR® Green Assay: CSE1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031938

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CSE1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CSE1L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018573
Assay Design:   Intron-spanning
Chromosome Location:   20:47675005-47679812question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000262982 ENST00000396192 ENST00000542325 ENST00000417408

Gene Information

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS while importin-beta mediates translocation through the nuclear pore complex. After translocation RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   CSE1L
Gene Name:   CSE1 chromosome segregation 1-like (yeast)
Aliases:   CAS, CSE1, MGC117283, MGC130036, MGC130037, XPO2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000124207
Entrez:   1434
Chromosome Mapping:   20q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download