PrimePCR™ SYBR® Green Assay: NME1-NME2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040232

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NME1-NME2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NME1-NME2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037509
Assay Design:   Intron-spanning
Chromosome Location:   17:49247352-49248930question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000393193 ENST00000555572 ENST00000393198 ENST00000393185 ENST00000393183 ENST00000514264 ENST00000513177 ENST00000512737 ENST00000503064 ENST00000393190 ENST00000270139 ENST00000299855 ENST00000572537

Gene Information

Description Not Available

Gene Symbol:   NME1-NME2
Gene Name:   NME1-NME2 readthrough
Aliases:   FLJ93990, NM23-LV, NMELV
RefSeq:   NC_000017.10 NG_021169.1 NT_010783.15
Ensembl:   ENSG00000243678
Entrez:   654364
Chromosome Mapping:   17q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.260000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download