PrimePCR™ lncRNA SYBR® Green Assay:ACTG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181761

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: ACTG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: ACTG1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0104521
Assay Design:   exonic
Chromosome Location:   chr17:81510166-81510308question
Amplicon Length:   113
Refseq Transcripts Amplified:   NM_001614,NM_001199954,NR_037688
Splice Variants Targeted:   ENST00000575842 ENST00000575087 ENST00000576544 ENST00000576209 ENST00000574671 ENST00000576917 ENST00000331925 ENST00000615544 ENST00000573283
LNCipedia Splice Variants Targeted:   lnc-ACTG1-2:2 lnc-ACTG1-2:3

Gene Information

Actins are highly conserved proteins that are involved in various types of cell motility and maintenance of the cytoskeleton. In vertebrates three main groups of actin isoforms alpha beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Actin gamma 1 encoded by this gene is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26 a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq Jan 2011]

Gene Symbol:   ACTG1
Gene Name:   actin, gamma 1
Aliases:   ACT, ACTG, DFNA20, DFNA26
RefSeq:   NC_000017.10 NG_011433.1 NT_010783.15
Ensembl:   ENSG00000184009
LNCipedia:   lnc-ACTG1-2
Entrez:   71
Chromosome Mapping:   17q25
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download