PrimePCR™ SYBR® Green Assay: Pdlim7, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043030

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Pdlim7, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Pdlim7, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040813
Assay Design:   exonic
Chromosome Location:   13:55508967-55512325question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000071880 ENSMUST00000081823 ENSMUST00000094135 ENSMUST00000126522 ENSMUST00000049346 ENSMUST00000103152 ENSMUST00000105487 ENSMUST00000105486 ENSMUST00000105485 ENSMUST00000105484 ENSMUST00000079751 ENSMUST00000120705 ENSMUST00000077407 ENSMUST00000113913 ENSMUST00000113919 ENSMUST00000113918 ENSMUST00000113907 ENSMUST00000165205 ENSMUST00000142618 ENSMUST00000159747 ENSMUST00000046246 ENSMUST00000069968 ENSMUST00000069929 ENSMUST00000131306 ENSMUST00000144288 ENSMUST00000153426

Gene Information

Description Not Available

Gene Symbol:   Pdlim7
Gene Name:   PDZ and LIM domain 7
Aliases:   1110003B01Rik, 2410002J21Rik, AV007930, Enigma
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000021493
Entrez:   67399
Chromosome Mapping:   13|13 B2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.960000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download