PrimePCR™ SYBR® Green Assay: Pcna, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043068

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Pcna, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Pcna, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040851
Assay Design:   exonic
Chromosome Location:   2:132252855-132253049question
Amplicon Length:   165
Splice Variants Targeted:   ENSMUST00000027393 ENSMUST00000031367 ENSMUST00000085809 ENSMUST00000031488 ENSMUST00000108017 ENSMUST00000109381 ENSMUST00000032327 ENSMUST00000111922 ENSMUST00000052011 ENSMUST00000167776 ENSMUST00000032629 ENSMUST00000085255 ENSMUST00000163845 ENSMUST00000088040 ENSMUST00000173433 ENSMUST00000038926 ENSMUST00000002368 ENSMUST00000171808 ENSMUST00000102710 ENSMUST00000102709 ENSMUST00000180142 ENSMUST00000087085 ENSMUST00000028817 ENSMUST00000122041 ENSMUST00000074103 ENSMUST00000120664 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Pcna
Gene Name:   Proliferating cell nuclear antigen
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027342
Entrez:   18538
Chromosome Mapping:   2 F2|2 75.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.000000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download