PrimePCR™ SYBR® Green Assay: Ptprc, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Ptprc, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Ptprc, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCID0004326
Assay Design:		intron_spanning
Chromosome Location:		13:60101478-60107711question
Amplicon Length:		120
Splice Variants Targeted:		ENSRNOT00000064785 ENSRNOT00000029878 ENSRNOT00000029865 ENSRNOT00000060292 ENSRNOT00000000814 ENSRNOT00000032587

Gene Information

member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

Gene Symbol:		Ptprc
Gene Name:		Receptor-type tyrosine-protein phosphatase C
Aliases:		Not Available
RefSeq:		NM_001109890 NM_001109889 NM_001109887 NM_001109888 NM_138507
Ensembl:		ENSRNOG00000000655
Entrez:		24699
Chromosome Mapping:		13q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.330000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.