PrimePCR™ SYBR® Green Assay: MEF2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: MEF2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MEF2A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015866
Assay Design:   Intron-spanning
Chromosome Location:   15:100246934-100250885question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000453228 ENST00000354410 ENST00000338042 ENST00000449277

Gene Information

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific growth factor-induced and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes including muscle development neuronal differentiation cell growth control and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jan 2010]

Gene Symbol:   MEF2A
Gene Name:   myocyte enhancer factor 2A
Aliases:   ADCAD1, RSRFC4, RSRFC9
RefSeq:   NC_000015.9 NT_010274.17 NG_016443.1
Ensembl:   ENSG00000068305
Entrez:   4205
Chromosome Mapping:   15q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 34.320000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download