PrimePCR™ SYBR® Green Assay: MRE11A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049279

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PrimePCR™ PreAmp for SYBR® Green Assay: MRE11A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MRE11A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042016
Assay Design:   exonic
Chromosome Location:   11:94219090-94219211question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000323929 ENST00000407439 ENST00000323977 ENST00000393241 ENST00000540013 ENST00000536754 ENST00000538923

Gene Information

This gene encodes a nuclear protein involved in homologous recombination telomere length maintenance and DNA double-strand break repair. By itself the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   MRE11A
Gene Name:   MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
Aliases:   ATLD, HNGS1, MRE11, MRE11B
RefSeq:   NC_000011.9 NT_167190.1 NG_007261.1
Ensembl:   ENSG00000020922
Entrez:   4361
Chromosome Mapping:   11q21

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