PrimePCR™ SYBR® Green Assay: TGM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0055668


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PrimePCR™ PreAmp for SYBR® Green Assay: TGM2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TGM2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0048408
Assay Design:		exonic
Chromosome Location:		20:36789890-36793599question
Amplicon Length:		91
Splice Variants Targeted:		ENST00000361475 ENST00000373403 ENST00000453095 ENST00000536701 ENST00000358775 ENST00000359373 ENST00000393605 ENST00000334211 ENST00000393609 ENST00000426523 ENST00000429686 ENST00000455638

Gene Information

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer is induced by retinoic acid and appears to be involved in apoptosis. Finally the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		TGM2
Gene Name:		transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Aliases:		G-ALPHA-h, GNAH, TG2, TGC
RefSeq:		NC_000020.10 NT_011362.10
Ensembl:		ENSG00000198959
Entrez:		7052
Chromosome Mapping:		20q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.080000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.