PrimePCR™ SYBR® Green Assay: IFNAR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: IFNAR2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IFNAR2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0006670
Assay Design:		Intron-spanning
Chromosome Location:		21:34617328-34619084question
Amplicon Length:		84
Splice Variants Targeted:		ENST00000382264 ENST00000404220 ENST00000342136 ENST00000342101 ENST00000413881 ENST00000443073 ENST00000447980 ENST00000382241

Gene Information

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases which in turn phosphorylate several proteins including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		IFNAR2
Gene Name:		interferon (alpha, beta and omega) receptor 2
Aliases:		IFN-R, IFN-alpha-REC, IFNABR, IFNARB
RefSeq:		NC_000021.8 NG_012089.1 NT_011512.11 NG_016003.1
Ensembl:		ENSG00000159110
Entrez:		3455
Chromosome Mapping:		21q22.1,21q22.11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	34.910000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.