PrimePCR™ SYBR® Green Assay: PFKM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011079
Assay Design:   Intron-spanning
Chromosome Location:   12:48524150-48525143question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000550345 ENST00000549003 ENST00000550924 ENST00000549941 ENST00000550257 ENST00000340802 ENST00000552792 ENST00000548288 ENST00000359794 ENST00000551339 ENST00000548345 ENST00000551804 ENST00000549022 ENST00000547587 ENST00000395233 ENST00000312352

Gene Information

Three phosphofructokinase isozymes exist in humans: muscle liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase which catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq Nov 2009]

Gene Symbol:   PFKM
Gene Name:   phosphofructokinase, muscle
Aliases:   GSD7, MGC8699, PFK-1, PFK1, PFKA, PFKX
RefSeq:   NC_000012.11 NT_029419.12 NG_016199.1
Ensembl:   ENSG00000152556
Entrez:   5213
Chromosome Mapping:   12q13.3

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