PrimePCR™ SYBR® Green Assay: CDKN1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028473


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PrimePCR™ PreAmp for SYBR® Green Assay: CDKN1B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CDKN1B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0012509
Assay Design:		Intron-spanning
Chromosome Location:		12:12871140-12871823question
Amplicon Length:		144
Splice Variants Targeted:		ENST00000228872 ENST00000535674

Gene Information

This gene encodes a cyclin-dependent kinase inhibitor which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes and thus controls the cell cycle progression at G1. The degradation of this protein which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq Jul 2008]

Gene Symbol:		CDKN1B
Gene Name:		cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Aliases:		CDKN4, KIP1, MEN1B, MEN4, P27KIP1
RefSeq:		NC_000012.11 NG_016341.1 NT_009714.17
Ensembl:		ENSG00000111276
Entrez:		1027
Chromosome Mapping:		12p13.1-p12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.660000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.