PrimePCR™ SYBR® Green Assay: PLCE1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: PLCE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLCE1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017826
Assay Design:   Intron-spanning
Chromosome Location:   10:95995755-96005756question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000371380 ENST00000371375 ENST00000260766 ENST00000371385

Gene Information

This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-45-bisphosphate to generate two second messengers: inositol 145-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth differentiation and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria edema and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq Sep 2009]

Gene Symbol:   PLCE1
Gene Name:   phospholipase C, epsilon 1
Aliases:   FLJ23659, KIAA1516, MGC167842, NPHS3, PLCE, PPLC
RefSeq:   NC_000010.10 NT_030059.13 NG_015799.1
Ensembl:   ENSG00000138193
Entrez:   51196
Chromosome Mapping:   10q23

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