PrimePCR™ SYBR® Green Assay: MGST1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0057878

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MGST1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MGST1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0056773
Assay Design:   Intron-spanning
Chromosome Location:   12:16507253-16516733question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000536371 ENST00000396210 ENST00000535309 ENST00000540126 ENST00000396207 ENST00000010404 ENST00000396209

Gene Information

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins two of which are involved in the production of leukotrienes and prostaglandin E important mediators of inflammation. Other family members demonstrating glutathione S-transferase and peroxidase activities are involved in cellular defense against toxic carcinogenic and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Four transcript variants of this gene encode one protein isoform. [provided by RefSeq Jul 2008]

Gene Symbol:   MGST1
Gene Name:   microsomal glutathione S-transferase 1
Aliases:   GST12, MGC14525, MGST, MGST-I
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000008394
Entrez:   4257
Chromosome Mapping:   12p12.3-p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.380000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download