PrimePCR™ SYBR® Green Assay: Gpx4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0060653

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gpx4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gpx4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052229
Assay Design:   exonic
Chromosome Location:   10:80056207-80056331question
Amplicon Length:   95
Splice Variants Targeted:   ENSMUST00000105372 ENSMUST00000097227

Gene Information

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide organic hydroperoxide and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation rat produces proteins that localize to the nucleus mitochondrion and cytoplasm. In mice experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Two pseudogenes exist for this gene one on chromosome 10 and one on chromosome 17. [provided by RefSeq Jul 2008]

Gene Symbol:   Gpx4
Gene Name:   Glutathione peroxidase 4
Aliases:   1700027O09Rik, PHGPx, mtPHGPx, snGPx
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000075706
Entrez:   625249
Chromosome Mapping:   10 C1|10 43.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download