PrimePCR™ SYBR® Green Assay: Fstl3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Fstl3, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Fstl3, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0021049
Assay Design:		Intron-spanning
Chromosome Location:		10:79778590-79779999question
Amplicon Length:		164
Splice Variants Targeted:		ENSMUST00000090219 ENSMUST00000102613 ENSMUST00000060441 ENSMUST00000113808 ENSMUST00000022220 ENSMUST00000171263 ENSMUST00000045097 ENSMUST00000110627 ENSMUST00000110628 ENSMUST00000052029 ENSMUST00000110626 ENSMUST00000110625 ENSMUST00000009099 ENSMUST00000109723 ENSMUST00000069690 ENSMUST00000109726 ENSMUST00000109727 ENSMUST00000061552 ENSMUST00000074570 ENSMUST00000166802 ENSMUST00000034316 ENSMUST00000035766 ENSMUST00000101670 ENSMUST00000025617 ENSMUST00000020575 ENSMUST00000085222 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Fstl3
Gene Name:		Follistatin-like 3
Aliases:		E030038F23Rik, Flrg
RefSeq:		NC_000076.6 NT_039500.8
Ensembl:		ENSMUSG00000020325
Entrez:		83554
Chromosome Mapping:		10 C1\|10

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.610000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.