PrimePCR™ SYBR® Green Assay: Ube2v1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043649

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ube2v1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ube2v1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041433
Assay Design:   Intron-spanning
Chromosome Location:   2:167610396-167617918question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000142742 ENSMUST00000102557 ENSMUST00000031590 ENSMUST00000180252 ENSMUST00000006618 ENSMUST00000004684 ENSMUST00000111785 ENSMUST00000111788 ENSMUST00000111784 ENSMUST00000099086 ENSMUST00000114242 ENSMUST00000025193 ENSMUST00000095347 ENSMUST00000109207 ENSMUST00000151365 ENSMUST00000140216 ENSMUST00000125544

Gene Information

Description Not Available

Gene Symbol:   Ube2v1
Gene Name:   Ubiquitin-conjugating enzyme E2 variant 1
Aliases:   0610011J09Rik, AI256840, CROC-1, CROC1, D7Bwg1382e, UEV-1
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000078923
Entrez:   66589
Chromosome Mapping:   2 H3|2 49.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 41.300000
Efficiency 82

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download