PrimePCR™ SYBR® Green Assay: RNF5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040023


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PrimePCR™ PreAmp for SYBR® Green Assay: RNF5, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RNF5, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0037300
Assay Design:		exonic
Chromosome Location:		6:32146331-32146423question
Amplicon Length:		63
Splice Variants Targeted:		ENST00000449794 ENST00000413786 ENST00000456167 ENST00000318962 ENST00000415953 ENST00000442544 ENST00000581580 ENST00000412726 ENST00000409790 ENST00000261657 ENST00000381822 ENST00000432616 ENST00000448838 ENST00000227868 ENST00000453473 ENST00000367137 ENST00000445885 ENST00000472060 ENST00000375094 ENST00000427134

Gene Information

The protein encoded by this gene contains a RING finger which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq Jul 2008]

Gene Symbol:		RNF5
Gene Name:		ring finger protein 5
Aliases:		RING5, RMA1
RefSeq:		NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:		ENSG00000204308
Entrez:		6048
Chromosome Mapping:		6p21.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	36.680000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.