PrimePCR™ SYBR® Green Assay: IFNGR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049300


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PrimePCR™ PreAmp for SYBR® Green Assay: IFNGR1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IFNGR1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042037
Assay Design:		exonic
Chromosome Location:		6:137525544-137527298question
Amplicon Length:		94
Splice Variants Targeted:		ENST00000367735 ENST00000543628 ENST00000414770 ENST00000458076 ENST00000367739

Gene Information

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq Jul 2008]

Gene Symbol:		IFNGR1
Gene Name:		interferon gamma receptor 1
Aliases:		CD119, FLJ45734, IFNGR
RefSeq:		NC_000006.11 NG_007394.1 NT_025741.15
Ensembl:		ENSG00000027697
Entrez:		3459
Chromosome Mapping:		6q23.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	1.000000
y-intercept	35.560000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.