PrimePCR™ SYBR® Green Assay: VCP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026398


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PrimePCR™ PreAmp for SYBR® Green Assay: VCP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: VCP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0007251
Assay Design:		Intron-spanning
Chromosome Location:		9:35066722-35067939question
Amplicon Length:		115
Splice Variants Targeted:		ENST00000358901 ENST00000448530 ENST00000417448

Gene Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion 26S proteasome function and assembly of peroxisomes. This protein as a structural protein is associated with clathrin and heat-shock protein Hsc70 to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis including homotypic membrane fusion spindle pole body function and ubiquitin-dependent protein degradation. [provided by RefSeq Jul 2008]

Gene Symbol:		VCP
Gene Name:		valosin containing protein
Aliases:		IBMPFD, MGC131997, MGC148092, MGC8560, TERA, p97
RefSeq:		NC_000009.11 NG_007312.1 NG_007887.1 NT_008413.18
Ensembl:		ENSG00000165280
Entrez:		7415
Chromosome Mapping:		9p13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998500
y-intercept	34.840000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.