PrimePCR™ SYBR® Green Assay: SEC61A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027834


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PrimePCR™ PreAmp for SYBR® Green Assay: SEC61A1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SEC61A1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0011040
Assay Design:		Intron-spanning
Chromosome Location:		3:127783810-127785888question
Amplicon Length:		133
Splice Variants Targeted:		ENST00000243253 ENST00000424880 ENST00000464451

Gene Information

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex which also contains beta and gamma subunits. [provided by RefSeq Jul 2008]

Gene Symbol:		SEC61A1
Gene Name:		Sec61 alpha 1 subunit (S. cerevisiae)
Aliases:		HSEC61, SEC61, SEC61A
RefSeq:		NC_000003.11 NT_005612.16
Ensembl:		ENSG00000058262
Entrez:		29927
Chromosome Mapping:		3q21.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997700
y-intercept	34.810000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.