PrimePCR™ SYBR® Green Assay: FCN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028672

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FCN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FCN2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012970
Assay Design:   Intron-spanning
Chromosome Location:   9:137778318-137779073question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000291744 ENST00000350339

Gene Information

The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide a short N-terminal segment followed by a collagen-like region and a C-terminal fibrinogen-like domain. This gene is predominantly expressed in the liver and has been shown to have carbohydrate binding and opsonic activities. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   FCN2
Gene Name:   ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
Aliases:   EBP-37, FCNL, P35, ficolin-2
RefSeq:   NC_000009.11 NG_011649.1 NT_019501.13
Ensembl:   ENSG00000160339
Entrez:   2220
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.200000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download