PrimePCR™ SYBR® Green Assay: SEC23B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033699

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEC23B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEC23B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023148
Assay Design:   Intron-spanning
Chromosome Location:   20:18535002-18541358question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000336714 ENST00000262544 ENST00000377475 ENST00000377465 ENST00000422877

Gene Information

The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq Feb 2010]

Gene Symbol:   SEC23B
Gene Name:   Sec23 homolog B (S. cerevisiae)
Aliases:   CDA-II, CDAII, CDAN2, HEMPAS
RefSeq:   NC_000020.10 NT_011387.8 NG_016281.1
Ensembl:   ENSG00000101310
Entrez:   10483
Chromosome Mapping:   20p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 35.200000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download