PrimePCR™ SYBR® Green Assay: GUCY1A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GUCY1A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GUCY1A3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023472
Assay Design:   Intron-spanning
Chromosome Location:   4:156625086-156631719question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000506455 ENST00000511108 ENST00000511507 ENST00000455639 ENST00000393832 ENST00000296518 ENST00000513574

Gene Information

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3'5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   GUCY1A3
Gene Name:   guanylate cyclase 1, soluble, alpha 3
Aliases:   GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164116
Entrez:   2982
Chromosome Mapping:   4q31.3-q33,4q31.1-q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download