PrimePCR™ SYBR® Green Assay: SEC61A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0039050


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PrimePCR™ PreAmp for SYBR® Green Assay: SEC61A2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SEC61A2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0036326
Assay Design:		Intron-spanning
Chromosome Location:		10:12185144-12191688question
Amplicon Length:		116
Splice Variants Targeted:		ENST00000379051 ENST00000441368 ENST00000298428 ENST00000304267 ENST00000379020 ENST00000379017 ENST00000457034 ENST00000418772 ENST00000379033

Gene Information

The protein encoded by this gene has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. It may also be required for the assembly of membrane and secretory proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]

Gene Symbol:		SEC61A2
Gene Name:		Sec61 alpha 2 subunit (S. cerevisiae)
Aliases:		FLJ10578
RefSeq:		NC_000010.10 NT_008705.16
Ensembl:		ENSG00000065665
Entrez:		55176
Chromosome Mapping:		10p14

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	1.000000
y-intercept	35.650000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.