PrimePCR™ SYBR® Green Assay: Pin1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Pin1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Pin1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020681
Assay Design:		Intron-spanning
Chromosome Location:		9:20655411-20662345question
Amplicon Length:		188
Splice Variants Targeted:		ENSMUST00000120196 ENSMUST00000119647 ENSMUST00000051777 ENSMUST00000098259 ENSMUST00000044271 ENSMUST00000103017 ENSMUST00000042078 ENSMUST00000047889 ENSMUST00000138694 ENSMUST00000006105 ENSMUST00000034689 ENSMUST00000036019 ENSMUST00000079158 ENSMUST00000115547

Gene Information

Description Not Available

Gene Symbol:		Pin1
Gene Name:		Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
Aliases:		0610025L01Rik, D9Bwg1161e
RefSeq:		NC_000075.6 NT_039472.8
Ensembl:		ENSMUSG00000032171
Entrez:		23988
Chromosome Mapping:		9 A3\|9 4.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	34.880000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.