PrimePCR™ SYBR® Green Assay: Hist1h2bc, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2bc, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist1h2bc, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021066
Assay Design:   Intron-spanning
Chromosome Location:   13:23684491-23692261question
Amplicon Length:   187
Splice Variants Targeted:   ENSMUST00000084852 ENSMUST00000163183 ENSMUST00000036018 ENSMUST00000020629 ENSMUST00000149179 ENSMUST00000108856 ENSMUST00000017458 ENSMUST00000100398 ENSMUST00000042035 ENSMUST00000048139 ENSMUST00000112793 ENSMUST00000075591 ENSMUST00000078320 ENSMUST00000112790 ENSMUST00000112825 ENSMUST00000018246 ENSMUST00000114001 ENSMUST00000113999 ENSMUST00000064797 ENSMUST00000114002 ENSMUST00000056482 ENSMUST00000113523 ENSMUST00000004201 ENSMUST00000092155 ENSMUST00000113122 ENSMUST00000113119 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist1h2bc
Gene Name:   Histone cluster 1, H2bc
Aliases:   2610022J01Rik, H2bfs, R74621
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000018102
Entrez:   68024
Chromosome Mapping:   13 A3.1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.790000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download