PrimePCR™ SYBR® Green Assay: Klk1b11, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Klk1b11, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Klk1b11, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024319
Assay Design:   Intron-spanning
Chromosome Location:   7:43997810-43998920question
Amplicon Length:   154
Splice Variants Targeted:   ENSMUST00000088696 ENSMUST00000160457 ENSMUST00000052699 ENSMUST00000099307 ENSMUST00000057561 ENSMUST00000100410 ENSMUST00000117878 ENSMUST00000097707 ENSMUST00000081154 ENSMUST00000163455 ENSMUST00000022947 ENSMUST00000029106 ENSMUST00000180222 ENSMUST00000166578 ENSMUST00000110510 ENSMUST00000094833 ENSMUST00000114113 ENSMUST00000007156 ENSMUST00000155534 ENSMUST00000163321 ENSMUST00000041924 ENSMUST00000046168 ENSMUST00000109470 ENSMUST00000073364 ENSMUST00000027753 ENSMUST00000117913 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Klk1b11
Gene Name:   Kallikrein 1-related peptidase b11
Aliases:   Klk11, mGK-11
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000044485
Entrez:   16613
Chromosome Mapping:   7 B4|7 23.16 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.400000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download