PrimePCR™ SYBR® Green Assay: Cftr, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Cftr, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Cftr, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0025170
Assay Design:   Intron-spanning
Chromosome Location:   6:18230089-18235201question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000045706 ENSMUST00000129452 ENSMUST00000115406

Gene Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This gene encodes the cystic fibrosis transmembrane regulator and a chloride channel that controls the regulation of other transport pathways. Mutations in this gene have been associated with autosomal recessive disorders such as cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternative splicing of exons 4 5 and 11 have been observed but full-length transcripts have not yet been fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   Cftr
Gene Name:   Cystic fibrosis transmembrane conductance regulator homolog
Aliases:   AW495489, Abcc7
RefSeq:   NC_000072.6 NT_039340.7
Ensembl:   ENSMUSG00000041301
Entrez:   12638
Chromosome Mapping:   6 A3|6 3.1 cM

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