PrimePCR™ SYBR® Green Assay: DUSP6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039306

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DUSP6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DUSP6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036582
Assay Design:   exonic
Chromosome Location:   12:89743092-89743203question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000547291 ENST00000308385 ENST00000279488

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2 is expressed in a variety of tissues with the highest levels in heart and pancreas and unlike most other members of this family is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP6
Gene Name:   dual specificity phosphatase 6
Aliases:   MKP3, PYST1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000139318
Entrez:   1848
Chromosome Mapping:   12q22-q23

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