PrimePCR™ SYBR® Green Assay: ACP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040770


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PrimePCR™ PreAmp for SYBR® Green Assay: ACP2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ACP2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038047
Assay Design:		exonic
Chromosome Location:		11:47269607-47270249question
Amplicon Length:		70
Splice Variants Targeted:		ENST00000485022 ENST00000256997 ENST00000529444 ENST00000533929 ENST00000529663 ENST00000530453 ENST00000444355 ENST00000529788 ENST00000537863 ENST00000561603 ENST00000304330 ENST00000367559 ENST00000444138 ENST00000539397

Gene Information

This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:		ACP2
Gene Name:		acid phosphatase 2, lysosomal
Aliases:		Not Available
RefSeq:		NC_000011.9 NT_009237.18 NG_009365.1 NG_023296.1
Ensembl:		ENSG00000134575
Entrez:		53
Chromosome Mapping:		11p11.2,11p12-p11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998700
y-intercept	35.360000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.