PrimePCR™ SYBR® Green Assay: MAP4K1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050294


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PrimePCR™ PreAmp for SYBR® Green Assay: MAP4K1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MAP4K1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043032
Assay Design:		exonic
Chromosome Location:		19:39088165-39090585question
Amplicon Length:		61
Splice Variants Targeted:		ENST00000591517 ENST00000589130 ENST00000396857 ENST00000591921 ENST00000423454

Gene Information

Description Not Available

Gene Symbol:		MAP4K1
Gene Name:		mitogen-activated protein kinase kinase kinase kinase 1
Aliases:		HPK1
RefSeq:		NC_000019.9 NG_008866.1 NT_011109.16
Ensembl:		ENSG00000104814
Entrez:		11184
Chromosome Mapping:		19q13.1-q13.4

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.790000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.