PrimePCR™ SYBR® Green Assay: NT5C3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051217

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NT5C3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NT5C3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043955
Assay Design:   exonic
Chromosome Location:   7:33057107-33059270question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000396152 ENST00000242210 ENST00000409467 ENST00000409787 ENST00000405342 ENST00000381626

Gene Information

This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq Mar 2012]

Gene Symbol:   NT5C3
Gene Name:   5'-nucleotidase, cytosolic III
Aliases:   MGC27337, MGC87109, MGC87828, P5'N-1, PN-I, PSN1, UMPH, UMPH1, cN-III
RefSeq:   NC_000007.13 NG_015800.1 NT_007819.17
Ensembl:   ENSG00000122643
Entrez:   51251
Chromosome Mapping:   7p14.3

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